Thermo Fisher Scientific Phospho-MEK2 (Thr394) Polyclonal Antibody
인산화된 MEK2(Thr394)를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot 및 IHC에 적합하며, Protein A로 정제된 액상 형태. 인간 시료에 반응하며, -20°C에서 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence that includes phosphorylation site of threonine (P-G-T(p)-P-T) derived from human MEK2 and conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | Dulbecco’s PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
MAP2K2 (MEK2) is a dual specificity serine/threonine kinase that plays a critical role in mitogen growth factor signal transduction. MEK2 (also known as MAPKK2) activates ERK1/2 and MAPK enzymes by phosphorylating both residues within the threonine/glutamate/tyrosine (TEY) motif in the activation loop. MEK1 and MEK2 are activated by dual phosphorylation on serine 218 and 222. Threonine 292 of MEK1 is phosphorylated by ERK2, serving as a negative feedback loop to suppress MEK1 activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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