Thermo Fisher Scientific Ep-CAM/CD326 (Extracellular Domain) (Epithelial Marker) Monoclonal Antibody (EGP40/1372), HRP
인간 Ep-CAM/CD326 단백질의 세포외 도메인을 인식하는 HRP 결합 단클론 항체. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용 가능. 세포 접착 및 종양 표지 연구용으로 적합. Protein A/G 정제, PBS 기반 저장.
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Thermo Fisher Scientific Ep-CAM/CD326 (Extracellular Domain) Monoclonal Antibody (EGP40/1372), HRP
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 0.5–1 µg/10^6 cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | EGP40/1372 |
| Immunogen | Recombinant fragment from the extracellular domain of human EpCAM protein (around aa100–224) |
| Conjugate | HRP |
| Form | Liquid |
| Concentration | 100 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | −20°C, avoid freeze/thaw cycles, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It functions as a calcium-independent cell adhesion molecule and influences cell cycle, proliferation, and metabolism through induction of c-myc, cyclin A, and cyclin E. Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression of Ep-CAM is associated with increased epithelial proliferation and is highly expressed in human carcinomas, serving as a marker for epithelial-derived tumors. It is found on the basolateral surface of simple epithelia and many carcinoma types, and helps distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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