Thermo Fisher Scientific CD266 Polyclonal Antibody
Rabbit polyclonal antibody against human Fn14 internal region. Suitable for IHC (paraffin) applications at 1:100 dilution. Recommended antigen retrieval with citrate buffer. Useful for studying TWEAK/TweakR signaling in tissue regeneration and angiogen...
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Internal region of human Fn14 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2549880 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
Suggested positive control tissues: placenta or kidney.
Target Information
TWEAK is a member of the TNF family and was first described as a weak inducer of apoptosis.
A receptor for TWEAK, known as TweakR, was isolated by expression cloning from a HUVEC cell cDNA library.
TweakR belongs to the TNF receptor superfamily and initiates proliferative signaling by binding to TRAF1, 2, and 3.
TweakR mRNA is upregulated during tissue regeneration in the aorta and liver and may play a role in vascular cell migration and angiogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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