Thermo Fisher Scientific Estrogen Receptor Beta Monoclonal Antibody (PPZ0506)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 6 publications 6 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

ELISA (ELISA)

0.2 µg/mL

-

Immunoprecipitation (IP)

Assay-Dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

PPZ0506

Immunogen

Estrogen Receptor beta antibody for 2-88 amino acid peptide in human ER beta. Ligand for estrogenic 17b-estradiol compound. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Estrogen Receptor beta,uniProtId:Q92731-1,ncbiNodeId:9606,antigenRange:2-88,antigenLength:530,antigenImageFileName:MA5-24807_Estrogen_Receptor_beta_Q92731-1_House_mouse.svg,antigenImageFileNamePDP:MA5-24807_Estrogen_Receptor_beta_Q92731-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

physiological saline

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2717280

Product Specific Information

This antibody specifically recognizes human ER beta but does not recognize human ER alpha.

Target Information

Estrogen Receptor beta (ER beta, NR3A2) protein is approximately 55kD, though multiple isoforms ranging from 36-59kD have been described. Human ER beta was initially cloned and characterized from testis. Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Estrogen receptor beta (ER beta) binds estrogens with an affinity similar to that of estrogen receptor alpha. Some ER beta isoforms dominantly inhibit the activity of estrogen receptor alpha in reproductive tissues. ER beta is found widely in many tissues throughout the body and can act as a potent tumor suppressor, playing a crucial role in many cancer types such as prostate cancer, autism spectrum disorder, medullary thyroid carcinoma, De Quervain Disease and risk of cardiovascular disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.