Thermo Fisher Scientific CD45.1 Monoclonal Antibody (A20), Biotin, eBioscience

Thermo Fisher Scientific CD45.1 Monoclonal Antibody (A20), Biotin, eBioscience™

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 532
• APC
• APC-eFluor 780
• Brilliant UV 395
• Brilliant UV 563
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 650
• Brilliant Violet 711
• Brilliant Violet 786
• eFluor 450
• eFluor 506
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 610-30S
• NovaFluor Blue 660-40S
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Request custom conjugation

Product Specific Information

The A20 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.1-expressing mouse strains (SJL/J, DA, STS/A, and RIII). CD45.1 is expressed by all leukocytes in these strains.

Applications Reported: Flow cytometric analysis and immunohistochemical staining of frozen tissue sections.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes at ≤0.25 µg per test (100 µL final volume). Cell number may vary from 10⁵–10⁸ cells/test. Antibody should be titrated for optimal performance.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising ~10% of lymphocyte surface proteins. It regulates Src-family kinases, crucial for lymphocyte development and antigen signaling. CD45 isoforms differ by extracellular domains due to alternative splicing and vary by cell type and physiological state. CD45 also modulates integrin-mediated signaling and participates in apoptosis-related DNA fragmentation. CD45RA is one isoform with restricted lymphoid cell expression.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.