Thermo Fisher Scientific CD45.2 Monoclonal Antibody (104), Biotin, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 395
• Brilliant UV 496
• Brilliant UV 563
• Brilliant UV 615
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 605
• Brilliant Violet 650
• Brilliant Violet 711
• Brilliant Violet 786
• eFluor 450
• eFluor 506
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 555
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 780
• Request custom conjugation

Product Specific Information

Description:
The 104 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.2-expressing mouse strains. These include C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A, and AKR strains. CD45.2 is expressed by all leukocytes in these strains.

Applications Reported:
The 104 antibody has been reported for use in flow cytometric analysis and immunohistochemical staining of frozen tissue sections.

Applications Tested:
The 104 antibody has been tested by flow cytometric analysis of mouse splenocytes. Recommended usage is ≤0.5 µg per test (100 µL final volume). Cell number should be determined empirically (10^5–10^8 cells/test). Optimal titration is recommended for each assay.

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising ~10% of surface proteins in lymphocytes. It is crucial for lymphocyte development and antigen signaling through regulation of Src-family kinases.
CD45.2 exists as multiple isoforms due to alternative splicing, differing in extracellular domains but sharing identical transmembrane and cytoplasmic regions. Isoform expression varies by cell type and physiological state.
Beyond immunoreceptor signaling, CD45.2 also promotes cell survival via integrin-mediated pathways and participates in DNA fragmentation during apoptosis. CD45RA is an isoform with restricted expression among lymphoid subsets.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.