Thermo Fisher Scientific Phospho-MEK5 (Ser311, Ser315) Polyclonal Antibody
MEK5 단백질의 Ser311 및 Ser315 인산화 부위를 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC, ELISA 등 다양한 응용에 사용 가능. Human, Mouse, Rat 반응성. Protein A로 정제된 1 mg/mL 액상 형태로, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human MEK5 around the phosphorylation site of Ser311 and Thr315 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
MAP2K5 (MEK5) is a serine/threonine kinase belonging to the MAP kinase family. It specifically interacts with and activates MAPK7/ERK5. MAP2K5 can be phosphorylated and activated by MAP3K3/MEKK3 and atypical protein kinase C isoforms (aPKCs). The MAP2K5-mediated signaling cascade is involved in growth factor–stimulated cell proliferation and muscle cell differentiation. The rs3743354 polymorphism in the MAP2K5 promoter may influence colorectal cancer risk.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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