Thermo Fisher Scientific P-Selectin Recombinant Rabbit Monoclonal Antibody (001)
인간 P-Selectin(CD62P)에 특이적인 재조합 토끼 단일클론 항체로 높은 특이성과 민감도를 제공. ICC/IF 및 ELISA에 적합하며, 동물 유래 성분이 없는 포뮬레이션으로 로트 간 일관성 우수. 보존제 무첨가, 장기 보관 시 -20°C 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | 1:5,000–1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 001 |
| Immunogen | Recombinant Human SELP/P Selectin protein (Met1–Ala771) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C (short term); at -20°C (long term), avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2786825 |
Additional Formats
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide (final concentration 0.05%–0.1%) to prevent contamination.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal clones for production.
Advantages include:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
This antibody specifically recognizes Human SELP/P Selectin.
Target Information
P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type I transmembrane glycoprotein involved in cell rolling regulation. It is stored in Weibel-Palade bodies of endothelial cells and α-granules of platelets, and rapidly translocates to the cell surface upon activation by thrombin, histamine, complement 5a, Ca²⁺ ionophores, or ADP.
P-selectin mediates interactions between activated endothelial cells or platelets and leukocytes, playing a crucial role in adhesion processes and inflammation. It binds to sialylated Lewis carbohydrate antigens on neutrophils and monocytes, contributing to atherogenesis, thrombosis, and tissue destruction. Clinically, P-selectin is used to differentiate heparin-induced thrombocytopenia with or without thrombosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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