Thermo Fisher Scientific CD100 Recombinant Rabbit Monoclonal Antibody (301)
Human CD100(SEMA4D) 인식하는 재조합 토끼 단클론 항체로, ELISA에 적합하며 높은 특이성과 민감도를 제공. 보존제 무첨가로 순도 높고, lot 간 일관성 우수. 단기 4°C, 장기 -20°C 보관 권장.
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Applications
Tested Application
- ELISA (ELISA): 1:5,000–1:10,000 dilution
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 301 |
| Immunogen | Recombinant Human Semaphorin 4D/SEMA4D/CD100 protein (Met1–Arg734) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2786832 |
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, and individual clones are screened for optimal performance.
Advantages of recombinant rabbit monoclonal antibodies:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to diverse rabbit immune repertoire
This antibody specifically recognizes Human Semaphorin 4D/SEMA4D/CD100.
Target Information
Semaphorins are neuronal chemorepellents guiding neuronal development.
CD100 (Semaphorin 4D) is a 150 kDa homodimer that induces B cell aggregation and enhances viability in vitro. It modulates CD40–CD40L signaling by promoting B cell aggregation and survival while downregulating CD23 expression.
CD72 acts as a lymphocyte receptor for CD100, regulating B cell signaling.
CD100 is expressed on resting and PHA-stimulated T cells, weakly on NK cells, EBV-transformed B cells, monocytes, and tumor/peripheral blood T cell lines, and more strongly on activated T and B cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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