Thermo Fisher Scientific p23 Monoclonal Antibody (JJ6), APC

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100

-

Immunocytochemistry (ICC/IF)

1:100

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Antibody Microarray (AM)

Assay-dependent

-

Product Specifications

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

JJ6

Immunogen

Recombinant human full length p23 protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:p23,uniProtId:Q15185-1,ncbiNodeId:9606,antigenRange:1-160,antigenLength:160,antigenImageFileName:MA5-45195_p23_Q15185-1_House_mouse.svg,antigenImageFileNamePDP:MA5-45195_p23_Q15185-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

0.5 µg/mL of MA5-45195 was sufficient for detection of p23 in 20 µg of heat shocked cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 23kDa.

Target Information

Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Prior to activation, steroid receptors associate with a number of different proteins in both a stable and transient fashion. Steroid receptor complex proteins include heat shock proteins (HSP70 and HSP90), immunosuppressant binding proteins called immunophilins (the FK506 binding proteins, FKBP52 & FKBP54 and the cyclosporin binding protein, CyP-40) and at least three other proteins termed p23, p60 and p48. p23 along with HSP70, HSP90 and p60, combine with progesterone receptor (PR) as members of a transient intermediate complex. Cloned human p23 encodes a protein of 160 amino acids that is highly conserved between species and shows no homology to previously identified proteins. p23 is a highly acidic phosphoprotein with an aspartic acid-rich C-terminal domain and multiple potential phosphorylation sites. In vitro studies have suggested that p23 binds to HSP90 and is necessary for the binding of HSP90 and CyP-40 to PR. While neither its exact function nor mechanism of action have been identified, p23 appears to be an important factor in PR function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.