Thermo Fisher Scientific HSC70 Monoclonal Antibody (1F2-H5), APC
HSC70 단백질을 인식하는 Thermo Fisher의 APC 결합 단일클론 항체로, Western blot, IHC, ICC 등 다양한 응용에 사용 가능. 인간, 마우스, 랫트 반응성. 단백질 G 정제, 보존제 없음, 4°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (IHC) | Assay-dependent | |
| Immunocytochemistry (ICC/IF) | 1:100 | |
| ELISA | Assay-dependent | |
| Immunoprecipitation (IP) | Assay-dependent | |
| Functional Assay | Assay-dependent | |
| in situ PLA (PLA) | Assay-dependent | |
| Immunomicroscopy (IM) | Assay-dependent | |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1F2-H5 |
| Immunogen | Full length human HSC70 (antigen range 1–646, UniProt ID: P11142-1) |
| Conjugate | APC (Allophycocyanin) |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931643 |
Additional Formats
- FITC (Cat. MA5-45190)
- PerCP (Cat. MA5-45191)
- Custom conjugation available upon request
Product Specific Information
- 1 µg/mL of MA5-45189 detects HSC70 in 10 µg of HeLa lysate by colorimetric immunoblot using Goat anti-mouse IgG:HRP secondary antibody.
- Detects approximately 73 kDa.
- Does not cross-react with HSP70.
Target Information
The HSP70 family consists of highly conserved proteins induced by various biological stresses, including heat shock, across many organisms.
Human HSP70 family members include:
- HSP70: Strongly inducible and constitutively expressed in primate cells
- HSP72: 72 kDa, stress-inducible
- HSC70 (HSC71, HSC73, HSP73, p72, prp73): 72 kDa, constitutively expressed, involved in clathrin-coated vesicle uncoating
- GRP78 (BiP): 78 kDa, ER-localized glucose-regulated protein
- p75 (HSP75): 75 kDa, mitochondrial localization
HSC70 binds to clathrin light chain loops to promote vesicle uncoating and interacts with the cytoskeleton, facilitating rearrangements. It also stimulates lysosomal degradation of intracellular proteins and modulates mitochondrial precursor protein folding.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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