Thermo Fisher Scientific Phospho-AKT1 (Tyr474) Polyclonal Antibody
Phospho-AKT1 (Tyr474) Polyclonal Antibody는 인간, 마우스, 랫트 등 다양한 종에 반응하며 세포 생존 및 신호전달 연구에 적합합니다. Western blot, IHC, ELISA에 사용 가능하며, 고순도 항원 친화 크로마토그래피로 정제된 제품입니다. 연구용으로만 사용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (IHC) | 1:100–1:300 |
| ELISA | 1:5,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthesized peptide derived from human Akt1 around the phosphorylation site of Y474 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2608974 |
Product Specific Information
This antibody is predicted to react with mouse, rat, and monkey based on sequence homology.
Target Information
AKT1 (PKB alpha) is a serine/threonine kinase regulating cell survival. Activated AKT1 inhibits apoptosis and promotes cell cycle progression by phosphorylating multiple targets across various cell types, including cancer cells. It is activated by insulin, PI3K, IGF1, and other growth factors, playing a crucial role in cell survival and cancer drug discovery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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