Thermo Fisher Scientific Phospho-p38 MAPK (Tyr323) Polyclonal Antibody
인간, 마우스, 랫트에 반응하는 Phospho-p38 MAPK (Tyr323) 폴리클로날 항체. Western blot, IHC, ELISA 등에 사용 가능. 항원 친화 크로마토그래피로 정제된 고순도 제품. PBS/BSA/glycerol 완충액에 보관, -20°C에서 안정적 저장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | - |
| Immunohistochemistry (IHC) | 1:100–1:300 | - |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| ELISA | 1:5,000 | - |
Product Specifications
| Category | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthesized peptide derived from human p38 around the phosphorylation site of Y323 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 0.5% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2607705 |
Product Specific Information
This antibody is predicted to react with mouse and rat based on sequence homology.
Target Information
MAPK14 (p38 alpha) is a member of the stress-activated protein kinase class of MAPKs. The p38 MAPK cascade regulates various cellular responses to stress, inflammation, and other stimuli. p38 MAPK is relatively inactive in its nonphosphorylated form and becomes rapidly activated by dual phosphorylation at Thr-Gly-Tyr motifs.
There are four isoforms of p38 MAPK that differ in tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other stimuli, MAPK kinase-3 is activated by phosphorylation. Activated MAPK kinase-3/6 phosphorylates Thr180 and Tyr182 residues in p38 MAPK. Phospho-p38 MAPK activates transcription factors such as ATF-2, CHOP-1, and MEF-2 through phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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