Thermo Fisher Scientific LDHA Polyclonal Antibody, Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:5,000

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

ELISA (ELISA)

1:5,000-1:20,000

-

Immunoprecipitation (IP)

1:100

-

Product Specifications

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Purified, full length, native Lactate Dehydrogenase.

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

10 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_794748

Product Specific Information

PA1-26911 is expected to cross react with a wide range of species due to sequence homology.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Lactate dehydrogenase (LDH) is the enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD+. In mammals, the enzyme is encoded by three genes: LDHA (M or muscle form), LDHB (H or heart form), and LDHC (X or testis form). The three human LDHs have 84-89% sequence similarities and 69-75% amino acid identities. There are five different LDH isoenzymes based on the proportion of M and H chains existing in the LDH tetrameric structure. The LDHA is hypoxia inducible and its expression is directly controlled by the transcriptional activity of the hypoxia inducible factor 1a (HIF1a). LDHB is also known to be upregulated in many cancers. Mutations in the LDHA gene can lead to Glycogen storage disease 11 (GSD11).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.