Thermo Fisher Scientific EpCAM Polyclonal Antibody, Biotin
EpCAM 단백질을 표적으로 하는 Biotin 결합 토끼 폴리클로날 항체. Western blot, ELISA, IP 등 다양한 응용에 적합. 인간 시료 반응성. Affinity chromatography로 정제된 고순도 항체로, 안정적인 보관 및 재현성 높은 결과 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | N-epitope specific synthetic peptide taken within amino acid region 100–200 on human Tumor-associated calcium signal transducer 1 protein (EpCAM, CD326; UniProt ID: P16422-1). |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Ep-CAM (epithelial adhesion molecule, also known as epithelial specific antigen, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It functions as a calcium-independent cell adhesion molecule influencing cell cycle, proliferation, and metabolism by inducing proto-oncogene c-myc and cyclins A and E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression of Ep-CAM is linked to increased epithelial proliferation and is a known marker for epithelial-derived tumors. It is highly expressed in human carcinomas and helps distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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