Thermo Fisher Scientific EpCAM Polyclonal Antibody, Biotin
EpCAM 단백질을 인식하는 Biotin 결합 토끼 폴리클로날 항체. Western blot, ELISA, IP 등에 사용 가능. 인간 시료에 반응하며, Affinity chromatography로 정제됨. -20°C에서 보관. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 215–265 of human Tumor-associated calcium signal transducer 1 (EpCAM, CD326; UniProt ID: P16422-1) |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Ep-CAM (epithelial adhesion molecule, ESA) is a transmembrane glycoprotein of approximately 40 kDa expressed in epithelial tissues. It functions as a Ca²⁺-independent cell adhesion molecule influencing cell cycle, proliferation, and metabolism by inducing c-myc and cyclins A/E. Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression is associated with enhanced epithelial proliferation and is a marker for epithelial-derived tumors. Ep-CAM is expressed on the basolateral surface of most simple epithelia and many carcinomas, distinguishing adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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