Thermo Fisher Scientific CD16/CD32 Monoclonal Antibody (93), Alexa Fluor 700, eBioscience

Thermo Fisher Scientific CD16/CD32 Monoclonal Antibody (Clone 93), Alexa Fluor 700

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• APC
• Biotin
• Brilliant UV 496
• Brilliant UV 661
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 710
• NovaFluor Red 755
• NovaFluor Yellow 690
• PE
• PE-Cyanine7
• PerCP-Cyanine5.5
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32.
CD16 (Fc gamma III Receptor) and CD32 (Fc gamma II Receptor) are low-affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils.

Applications Reported:
This 93 antibody has been reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended usage ≤ 0.25 µg per test (100 µL volume, 10⁵–10⁸ cells/test). Antibody should be titrated for optimal performance.

Fluorophore Information:
Alexa Fluor® 700 emits at 723 nm and is excited with the red laser (633 nm). Use 685 LP mirror and 710/20 filter for detection.

Optical Properties:

• Excitation: 633–647 nm
• Emission: 723 nm
• Laser: Red Laser

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

CD16 is a 50–65 kDa cell surface molecule expressed in two forms:

• Transmembranous form (NK cells, some T cells)
• Phosphatidylinositol-linked form (granulocytes)

CD16 acts as a low-affinity receptor for IgG (FcR III) and mediates ADCC (antibody-dependent cell-mediated cytotoxicity).

CD32 is a 40 kDa glycoprotein functioning as a low-affinity receptor for IgG (Fc gamma RII). It mediates endocytosis, activation of secretion, cytotoxicity, and immunomodulation.
Expressed by B cells, monocytes, granulocytes, and platelets.
Involved in immune complex phagocytosis and regulation of antibody production by B cells.
CD32 gene variations may increase susceptibility to systemic lupus erythematosus (SLE).
Several transcript variants encoding different isoforms of CD32 have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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