Thermo Fisher Scientific CD16/CD32 Monoclonal Antibody (93), Alexa Fluor 700, eBioscience
마우스 CD16/CD32 항원을 인식하는 Rat IgG2a 단클론 항체로 Alexa Fluor 700 형광 표지. 유세포분석에 최적화되어 있으며 적색 레이저(633 nm)로 여기. 0.25 µg/test 권장, 4°C 암소 보관. 연구용으로만 사용.
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Thermo Fisher Scientific CD16/CD32 Monoclonal Antibody (Clone 93), Alexa Fluor 700
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | View 33 publications |
| Neutralization (Neu) | - | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fruit fly, Human, Mouse |
| Host / Isotype | Rat / IgG2a, lambda |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 700, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 93 |
| Conjugate | Alexa Fluor™ 700 |
| Excitation / Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_493995 |
Available Formats
- Unconjugated
- APC
- Biotin
- Brilliant UV 496
- Brilliant UV 661
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Red 685
- NovaFluor Red 700
- NovaFluor Red 710
- NovaFluor Red 755
- NovaFluor Yellow 690
- PE
- PE-Cyanine7
- PerCP-Cyanine5.5
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Request custom conjugation
Product Specific Information
The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32.
CD16 (Fc gamma III Receptor) and CD32 (Fc gamma II Receptor) are low-affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils.
Applications Reported:
This 93 antibody has been reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended usage ≤ 0.25 µg per test (100 µL volume, 10⁵–10⁸ cells/test). Antibody should be titrated for optimal performance.
Fluorophore Information:
Alexa Fluor® 700 emits at 723 nm and is excited with the red laser (633 nm). Use 685 LP mirror and 710/20 filter for detection.
Optical Properties:
- Excitation: 633–647 nm
- Emission: 723 nm
- Laser: Red Laser
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
CD16 is a 50–65 kDa cell surface molecule expressed in two forms:
- Transmembranous form (NK cells, some T cells)
- Phosphatidylinositol-linked form (granulocytes)
CD16 acts as a low-affinity receptor for IgG (FcR III) and mediates ADCC (antibody-dependent cell-mediated cytotoxicity).
CD32 is a 40 kDa glycoprotein functioning as a low-affinity receptor for IgG (Fc gamma RII). It mediates endocytosis, activation of secretion, cytotoxicity, and immunomodulation.
Expressed by B cells, monocytes, granulocytes, and platelets.
Involved in immune complex phagocytosis and regulation of antibody production by B cells.
CD32 gene variations may increase susceptibility to systemic lupus erythematosus (SLE).
Several transcript variants encoding different isoforms of CD32 have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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