Gibco™

RPMI 1640 Medium, no glutamine

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial자세히 알아보기

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. Life Technologies offers a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI is modified as follows:

With			Without
• Phenol Red			• HEPES
			• L-glutamine

The complete formulation is available.

Using RPMI
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagles Minimal Essential Medium or Dulbeccos Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Product intended use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco™ RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference our Type II Drug Master File (DMF).

cGMP manufacturing and quality system
RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, Life Technologies offers an identical RPMI 1640 product made in our Grand Island facility (21870-092). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

사양

배양 환경CO2

세포 유형Mammalian Cells

분류Animal Origin-free

농도1 X

Culture TypeMammalian Cell Culture

형태Liquid

제품 유형RPMI 1640

Serum LevelStandard Serum Supplementation

유통 기한12 Months

멸균Sterile-filtered

첨가제 포함Phenol Red, Sodium Bicarbonate

첨가제 없음No Glutamine, No HEPES, No Sodium Pyruvate

제조 품질cGMP-compliant under the ISO 13485 standard

삼투압260 - 310 mOsm/kg

pH7 to 7.4

제품라인Gibco™

수량500 mL

배송 조건Room Temperature

Unit SizeEach