Thermo Fisher Scientific HIF1AN Monoclonal Antibody (FIH 162c)
HIF1AN 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, WB, IHC(P), ICC/IF에 사용 가능. 인간 FIH 단백질을 면역원으로 제작. 비결합형 액상 형태이며, 1 mg/mL 농도. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:100–1:500 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | - |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | FIH 162c |
| Immunogen | Full length human FIH expressed in E. coli BL21 (DE3) cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2735217 |
Target Information
Hydroxylates HIF-1 alpha at Asn-803 in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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