Thermo Fisher Scientific GATA3 Monoclonal Antibody (UMAB218), UltraMAB
GATA3 단백질을 인식하는 Mouse Monoclonal Antibody (Clone UMAB218). Western blot, IHC, ICC/IF, ChIP, Peptide Array 등 다양한 응용에 적합. Human 시료에 반응하며, 고순도 Affinity chromatography로 정제된 Lyophilized 형태.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | 1:2,000 |
| ChIP Assay (ChIP) | Assay-dependent |
| Peptide Array (Array) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UMAB218 |
| Immunogen | Human recombinant protein fragment (aa 155–443 of human GATA3, produced in E. coli) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
GATA3 is a zinc finger transcription factor that binds enhancer elements of T-cell receptor (TCR) genes (alpha, beta, gamma, delta), regulating their expression. It is abundantly expressed in T-lymphocytes and involved in T-cell receptor gene activation. The human GATA3 gene comprises six exons spanning 17 kb, with two zinc fingers encoded by separate exons highly conserved with those of GATA1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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