Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio17B7), APC, eBioscience
Mouse 및 Rat IL-17A를 특이적으로 인식하는 단클론 항체로, Flow Cytometry 및 Intracellular Staining에 최적화됨. APC 형광물질로 결합되어 높은 감도 제공. 염증 반응 연구 및 Th17 세포 분석에 적합. 4°C 보관, 동결 금지.
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Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio17B7), APC, eBioscience™
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | View 111 publications |
| Functional Assay | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Mouse, Rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio17B7 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_763580 |
Available Formats
- Alexa Fluor 488
- Biotin
- Brilliant UV 395, 615, 661, 737, 805
- Brilliant Violet 421, 650, 711, 786
- eFluor 450, 506, 660
- FITC
- PE, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Custom conjugation available
Product Specific Information
The eBio17B7 antibody reacts with mouse and rat IL-17A, showing no cross-reactivity with IL-17F.
IL-17A is a CD4⁺ T cell-derived cytokine that promotes inflammatory responses and is elevated in autoimmune and inflammatory conditions such as rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection.
High levels of IL-17A homodimer are produced by activated peripheral blood CD4⁺ T cells, enhancing ICAM-1 expression in fibroblasts and stimulating secretion of IL-6, IL-8, G-CSF, and PGE2.
IL-17A also supports proliferation and maturation of CD34⁺ hematopoietic progenitors into neutrophils.
IL-23-dependent, IL-17A-producing CD4⁺ T cells (Th17 cells) represent a distinct subset of helper T cells. IFN-γ and IL-4 negatively regulate Th17 cell generation.
Applications Reported: Useful for intracellular staining and flow cytometric analysis.
Applications Tested: Tested by intracellular staining and flow cytometry of restimulated, Th17-polarized mouse splenocytes using the Intracellular Fixation and Permeabilization Buffer Set (Product #88-8824-00). Recommended ≤0.125 µg/test.
Cell number per test may vary (10⁵–10⁸ cells). Optimize antibody titration for best performance.
Compatible with Foxp3 buffer system (Product #00-5523-00).
Excitation: 633–647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Interleukin-17A (IL-17A, CTLA-8) is a 32 kDa disulfide-linked homodimer consisting of 136 amino acids, part of the IL-17 family (IL-17A–F). It signals through the IL-17 receptor (IL-17R/CDw217).
IL-17A regulates NF-κB and MAPK pathways, stimulates IL-6 and COX-2 expression, and enhances nitric oxide (NO) production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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