Thermo Fisher Scientific CRYAB Monoclonal Antibody (1A7.D5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:100

-

ELISA (ELISA)

Assay-Dependent

-

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Pig, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1A7.D5

Immunogen

Native Human Alpha B Crystallin if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CRYAB,uniProtId:P02511-1,ncbiNodeId:9606,antigenRange:1-175,antigenLength:175,antigenImageFileName:MA5-27708_CRYAB_P02511-1_House_mouse.svg,antigenImageFileNamePDP:MA5-27708_CRYAB_P02511-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• APC
• FITC
• PE
• PerCP
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2735156

Product Specific Information

0.5 µg/mL of MA5-27708 was sufficient for detection of 50 ng purified alpha B crystalline by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 20kDa (Predicted mol. weight is approximately 21kDa). Does not cross-react with alpha A-crystallin, beta L-crystallin, beta H-crystallin, gamma -crystallin, HSP25, HSP27 or HSP47 proteins.

Target Information

Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products-alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander`s disease where it accumulates in brain cells of those afflicted.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.