Thermo Fisher Scientific HCN2 Monoclonal Antibody (S71)
HCN2 단백질을 특이적으로 인식하는 Thermo Fisher Scientific의 단클론 항체입니다. Western blot, IHC, ICC/IF 등 다양한 응용에 사용 가능하며, Human, Mouse, Rat 시료에 반응합니다. 단백질 G 정제, 액상 형태, -20°C 보관 조건입니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
| Antibody Microarray (AM) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | S71 |
| Immunogen | Fusion protein amino acids 761–863 of rat HCN2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735211 |
Additional Formats
Product Specific Information
1 µg/mL of MA5-27718 was sufficient for detection of HCN2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 95 kDa. No cross-reactivity against HCN1.
Target Information
Hyperpolarization-activated cation channels of the HCN gene family, such as HCN2, contribute to spontaneous rhythmic activity in both heart and brain. HCN2 is a member of a family of pacemaker channels activated by hyperpolarization and regulated by cyclic nucleotides. HCN1 and HCN2 play important roles in motor learning and neuronal integration by cerebellar Purkinje cells, as well as shaping autonomous activity of single neurons and the periodicity of network oscillations.
HCN1 expression is highly enriched in cerebral cortex, hippocampus, cerebellum, and facial motor nucleus, while HCN2 is abundant in mamillary bodies, pontine nucleus, ventral cochlear nucleus, and nucleus of the trapezoid body. These regional differences in HCN channel expression may contribute to variations in neuronal pacemaker activity across brain systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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