Thermo Fisher Scientific c-Met Recombinant Rabbit Monoclonal Antibody (22H22L13)
Thermo Fisher Scientific의 c-Met Recombinant Rabbit Monoclonal Antibody (22H22L13)은 인간 MET 단백질을 표적하는 고특이성 항체입니다. Western blot, ICC/IF, ELISA에 적합하며, 재조합 기술로 생산되어 높은 로트 간 일관성과 민감도를 제공합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:5,000 | View 2 publications |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | – |
| ELISA | 1–5 µg/mL | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 22H22L13 |
| Immunogen | Peptide corresponding to amino acids 1379–1390 of UniProt ID P08581 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at –20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2532310 |
Product Specific Information
- Predicted to react with primates based on sequence homology.
- Intact IgG appears as a ~150 kDa band on non-reducing gel; upon reduction, generates ~25 kDa light chain and ~50 kDa heavy chain bands.
- Produced using in vitro recombinant expression systems derived from immunoreactive rabbit antibody DNA sequences.
- Advantages include improved specificity and sensitivity, lot-to-lot consistency, animal origin-free formulation, and broader immunoreactivity due to rabbit immune repertoire.
Target Information
MET (c-Met) is a receptor-like tyrosine kinase associated with various human malignancies. Upon ligand activation, MET interacts with PI3K subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3, triggering downstream signaling pathways such as RAS-ERK, PI3K-AKT, and PLCγ-PKC.
MET plays critical roles in:
- Embryonic development (gastrulation, muscle and neuron development, angiogenesis, kidney formation)
- Adult processes (wound healing, organ regeneration, tissue remodeling)
Dysregulation of MET is linked to cancers including gastric, renal, and breast, making it a key target for cancer research and diagnostics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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