Thermo Fisher Scientific XPG Polyclonal Antibody
ERCC5(XPG) 단백질을 인식하는 토끼 폴리클로날 항체. Western blot, IHC, ICC/IF에 사용 가능. 사람, 마우스, 랫트 반응성. 액상 형태, 1 mg/mL, PBS + 50% glycerol에 보관. DNA 손상 복구 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human ERCC5 (Accession P28715), corresponding to amino acid residues E140–S190 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2851842 |
Product Specific Information
Antibody detects endogenous levels of total ERCC5.
Target Information
Xeroderma pigmentosum type G (XPG) is a human genetic disease exhibiting extreme sensitivity to sunlight. The XPG protein, a member of the flap endonuclease 1 (FEN-1) structure-specific DNA repair endonuclease family, is an enzyme essential for DNA repair of solar ultraviolet (UV)-induced DNA damages. Human XPG nuclease makes the 3′ incision during nucleotide excision repair of DNA. The enzyme cleaves model DNA bubble structures near the junction of unpaired DNA with a duplex region. A 29-amino acid region of human XPG (residues 981–1009) contains the PCNA binding activity. A conserved arginine in XPG (Arg992) is crucial for its PCNA binding activity. Replication Protein A (RPA) binds specifically and directly to two excision repair proteins, the xeroderma pigmentosum damage-recognition protein XPA and the endonuclease XPG.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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