Thermo Fisher Scientific Phospho-SMURF1 (Ser200) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Product Specifications

Product Specific Information

Antibody detects endogenous levels of SMURF1 only when phosphorylated at Ser200.

Target Information

Members of the transforming growth factor-beta (TGFB) family signal through type I and type II serine/threonine-kinase receptors, which activate the SMAD signaling pathway.
Bone morphogenetic protein (BMP) receptors target SMAD1, SMAD5, and SMAD8, whereas receptors for activin and TGFB (e.g., ACVR1 and TGFBR1) target SMAD2 and SMAD3.
Phosphorylation of these receptor-regulated SMADs induces their association with the common-partner SMAD, SMAD4.

Smurf1, a HECT domain E3 ubiquitin ligase, regulates cell polarity and protrusive activity and is required to maintain the transformed morphology and motility of tumor cells.
Atypical protein kinase C-zeta (PKCζ), an effector of the Cdc42/Rac1–PAR6 polarity complex, recruits Smurf1 to cellular protrusions, where it controls the local level of RhoA.
Smurf1 thus links the polarity complex to degradation of RhoA in lamellipodia and filopodia to prevent RhoA signaling during dynamic membrane movements.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.