Thermo Fisher Scientific HMGB1 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Positive Control: HepG2, MCF-7, F9, A549, PC12, HeLa, NIH/3T3, RAW264.7, mouse kidney tissue, mouse stomach tissue, mouse brain tissue, human stomach carcinoma tissue, human tonsil tissue
• Subcellular Location: Nucleus

Target Information

HMGB1 (High-mobility group box-1) protein is a nuclear non-histone DNA-binding chromosomal protein. Damaged or necrotic cells release HMGB1 into the extracellular space, where it acts as a proinflammatory cytokine.
Mouse HMGB1 consists of 215 amino acids forming three domains: two positively charged DNA-binding domains (HMG box A, aa 9–79; box B, aa 89–162), and a negatively charged 30 aa C-terminal acidic tail.
Residues 28–44 and 180–185 contain nuclear localization signals (NLS). The B box mediates cytokine activity, while the A box interacts with transcription factors.

HMGB1 functions as:

• An inflammatory mediator promoting monocyte migration and cytokine secretion.
• A mediator of T cell–dendritic cell interaction.
• A signal transducer through receptors RAGE, TLR2, and TLR4.

Highly conserved and ubiquitous, HMGB1 is essential in inflammation during sterile and infection-associated responses. It is also implicated in trauma, sepsis, arthritis, and ischemia-reperfusion injury models.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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