Thermo Fisher Scientific HMGB1 Polyclonal Antibody
HMGB1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체로, WB, IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합. 인간, 마우스, 랫트 반응성. 고순도 Affinity 정제, 안정적 PBS/glycerol buffer 구성. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within human HMGB1 (aa 71–126) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 40% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2913200 |
Product Specific Information
- Positive Control: HepG2, MCF-7, F9, A549, PC12, HeLa, NIH/3T3, RAW264.7, mouse kidney tissue, mouse stomach tissue, mouse brain tissue, human stomach carcinoma tissue, human tonsil tissue
- Subcellular Location: Nucleus
Target Information
HMGB1 (High-mobility group box-1) protein is a nuclear non-histone DNA-binding chromosomal protein. Damaged or necrotic cells release HMGB1 into the extracellular space, where it acts as a proinflammatory cytokine.
Mouse HMGB1 consists of 215 amino acids forming three domains: two positively charged DNA-binding domains (HMG box A, aa 9–79; box B, aa 89–162), and a negatively charged 30 aa C-terminal acidic tail.
Residues 28–44 and 180–185 contain nuclear localization signals (NLS). The B box mediates cytokine activity, while the A box interacts with transcription factors.
HMGB1 functions as:
- An inflammatory mediator promoting monocyte migration and cytokine secretion.
- A mediator of T cell–dendritic cell interaction.
- A signal transducer through receptors RAGE, TLR2, and TLR4.
Highly conserved and ubiquitous, HMGB1 is essential in inflammation during sterile and infection-associated responses. It is also implicated in trauma, sepsis, arthritis, and ischemia-reperfusion injury models.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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