Thermo Fisher Scientific KCNMB1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

2.0 µg/mL

View 6 publications 6 publications

Immunohistochemistry (IHC)

-

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1:100-1:200

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Dog, Human, Mouse, Rabbit, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: L(90) Y H T E D T R D Q N Q Q C(103) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:KCNMB1,uniProtId:Q16558-1,ncbiNodeId:9606,antigenRange:90-103,antigenLength:191,antigenImageFileName:PA1-924_KCNMB1_Q16558-1_Rabbit.svg,antigenImageFileNamePDP:PA1-924_KCNMB1_Q16558-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2249548

Product Specific Information

PA1-924 detects large conductance, calcium-activated potassium channel beta (Maxi K+ Beta) from human, mouse, and rat samples.

PA1-924 has been successfully used in western blot and immunofluorescence. By western blot, this antibody detects an ~28kDa protein representing Maxi K+ beta from mouse myometrial smooth muscle cell extracts. The molecular weight detected varies due to glycosylation, multimerization and binding to actin.

Immunizing peptide corresponds to amino acid residues 90-103 from human Maxi K+ beta. This sequence is completely conserved across many species. PA1-924 immunizing peptide (Cat. # PEP-119) is available for use in neutralization and control experiments.

Target Information

Potassium channels are a group of ubiquitously expressed proteins that serve numerous functions in excitable and non-excitable cells. One class of integral membrane potassium channels is the large conductance, calcium-activated potassium channel (Maxi K+). Maxi K+ differs from most other potassium channels in that its activation is controlled by both increases in intracellular calcium and by membrane depolarization. Maxi K+ dual activation is possible because of its structure. The core of the channel, which is similar to other potassium channels, is a Maxi K+ alpha homotetramer that contains both a voltage sensor and an intracellular calcium binding domain. In vascular smooth muscle, an auxiliary beta-subunit is found in a 1:1 stoichiometry. The beta-subunit exhibits its effect on the Maxi K+ channel by effectively decreasing by 5- to 10- fold the concentration of calcium required to keep the pore open. Maxi K+ beta is the target for possible therapeutics because of its role in blood flow and blood pressure regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.