Thermo Fisher Scientific KCNMA1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

2.0 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

10.0 µg/mL

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C T(945) E L V N D T N V Q F L D Q D D D(961). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:KCNMA1,uniProtId:Q12791-1,ncbiNodeId:9606,antigenRange:945-961,antigenLength:1236,antigenImageFileName:PA1-923_KCNMA1_Q12791-1_Rabbit.svg,antigenImageFileNamePDP:PA1-923_KCNMA1_Q12791-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2234230

Product Specific Information

PA1-923 detects large conductance, calcium-activated potassium channel alpha (Maxi K+ alpha) from human tissues as well as recombinant human protein and rat samples.

PA1-923 has been successfully used in Western blot and immunocytochemistry procedures. By Western blot, this antibody detects an ~110 kDa protein representing Maxi K+ alpha from human myometrial smooth muscle cell extracts. Immunocytochemical staining of Maxi K+ alpha in human coronary muscle cells with PA1-923 results in predominant staining of the plasma membrane.

PA1-923 immunizing peptide corresponds to amino acid residues 945-961 from human Maxi K+ alpha. This sequence is completely conserved across many species. PA1-923 immunizing peptide (Cat. # PEP-113) is available for use in neutralization and control experiments.

Target Information

Potassium channels are a group of ubiquitously expressed proteins that serve numerous functions in excitable and non-excitable cells. One class of integral membrane potassium channels is the large conductance, calcium-activated potassium channel (Maxi K+). Maxi K+ differs from most other potassium channels in that its activation is controlled by both increases in intracellular calcium and by membrane depolarization. Maxi K+ dual activation is possible because of its structure. The core of the channel, which is similar to other potassium channels, is a Maxi K+ alpha homotetramer that contains both a voltage sensor and an intracellular calcium binding domain. In vascular smooth muscle, an auxiliary beta-subunit is found in a 1:1 stoichiometry. The beta-subunit exhibits its effect on the Maxi K+ channel by effectively decreasing by 5- to 10- fold the concentration of calcium required to keep the pore open.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.