Thermo Fisher Scientific PI3 Kinase p55 gamma Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (IHC)

1:100

-

Immunocytochemistry (ICC/IF)

15 µg/mL

-

ELISA (ELISA)

1:10,000-1:50,000

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Anti-PI3 Kinase p55 gamma antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-Terminal portion of human PI3 Kinase p55 gamma conjugated to Keyhole Limpet Hemocyanin (KLH). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PI3K p55 gamma,uniProtId:Q92569-1,ncbiNodeId:9606,antigenRange:1,antigenLength:461,antigenImageFileName:600-101-ML2_PI3K_p55_gamma_Q92569-1_Goat.svg,antigenImageFileNamePDP:600-101-ML2_PI3K_p55_gamma_Q92569-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.02 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against human PI 3 Kinase p55 gamma. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to mouse and rat, 94.1% homology to bovine.

Target Information

PIK3R3 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1. It is a component of a heterodimer of p110 (catalytic) and p55 (regulatory) subunits. The protein is expressed at highest levels in brain and testis. Lower levels are detected in adipose tissue, kidney, heart, lung and skeletal muscle. The protein contains 2 SH2 domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.