Thermo Fisher Scientific Brn3b Polyclonal Antibody
Goat-derived polyclonal antibody targeting mouse Brn3b (POU4F2), validated for WB, IHC, ICC/IF, and ELISA. High specificity and affinity-purified for reliable detection. Suitable for mouse and rat samples, stored at -20°C for long-term stability.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:100 |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| ELISA | 1:10,000–1:50,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a near N-terminal portion of mouse Pou4f2 conjugated to Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.05 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against mouse Brn3b. A BLAST analysis suggests cross-reactivity with rat (100% homology) and human (91.7% homology).
Target Information
POU4F2 (Brn3b) is a member of the POU-domain family of transcription factors involved in regulating cell identity.
In the human retina, POU4F2 is expressed in a subset of ganglion cells, playing a role in determining or maintaining the identities of specific visual system neurons.
[Supplied by OMIM]
Publication Note: This RefSeq record includes a subset of publications available for this gene. Please refer to the Entrez Gene record for additional references.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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