Thermo Fisher Scientific CD69 Monoclonal Antibody (H1.2F3), FITC, eBioscience
마우스 CD69 항원을 인식하는 FITC 결합 단클론 항체로, T 및 B 세포 활성화 연구에 적합합니다. Flow cytometry에 최적화되었으며 0.5 µg/test로 사용 권장됩니다. 488 nm에서 여기, 520 nm에서 방출하며 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 0.5 µg/test | 108 publications |
| ELISA | - | 1 publication |
| In vitro Assay (IV) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | H1.2F3 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465118 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 496 / 661 / 737
- Brilliant Violet 421 / 786
- eFluor 450
- Functional Grade
- PE
- PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436 / 600 / 645
- Request custom conjugation
Product Specific Information
The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 (~35 kDa) is expressed as a disulfide-linked dimer on lymphocyte surfaces. Activation of T and B cells rapidly increases CD69 expression, indicating its role in lymphocyte development and activation.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on resting and activated mouse splenocytes at ≤0.5 µg/test (100 µL final volume). Optimal titration is recommended.
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide-bonded homodimer (~60 kDa under non-reducing conditions). It is present on activated platelets and lymphocytes, playing a role in signal transduction and proliferation of T lymphocytes. CD69 expression is induced upon activation and may also participate in natural killer cell and platelet signaling.
Associated diseases include coccidiodomycosis and asthma.
Alternative splicing produces multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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