Thermo Fisher Scientific Alpha A Crystallin Monoclonal Antibody (1H3.B8)

Applications

Western Blot (WB)

• Tested Dilution: 0.5–1 µg/mL

ELISA

• Tested Dilution: Assay-dependent

Product Specifications

Target Information

Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found in vertebrate skeletal muscle tissue.
In the lens, their structural function is to assist in maintaining the proper refractive index of the lens.
The mammalian lens contains three major classes of crystallins: alpha, beta, and gamma.

• Alpha-crystallin is the largest of the crystallins and composed of two primary gene products: alpha-A and alpha-B.
• Beta-crystallins comprise at least five different proteins.
• Gamma-crystallins are monomeric, with at least five identified in bovine and rat lenses.

Alpha-crystallin comprises approximately 40% of total lens protein composition.
In addition to maintaining proper refractive index, it also functions in a chaperone-like manner by preventing the formation of aggregates that may lead to cataract formation.
Phosphorylated states of alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance.
Alpha-B crystallin has been linked to Alexander’s disease, where it accumulates in brain cells of affected individuals.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.