Thermo Fisher Scientific MAPK1 Monoclonal Antibody (OTI7E7), TrueMAB
MAPK1(ERK2)를 인식하는 Mouse 모노클로날 항체로 Western blot, ICC/IF, IP에 적합. Human, Dog, Rat 반응성. 동결건조형으로 PBS buffer에 8% 트레할로스 포함. 연구용으로 세포 신호전달 관련 연구에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| Immunoprecipitation (IP) | 2 µg/500 µL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI7E7 |
| Immunogen | Full length human recombinant protein of human MAPK1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20 °C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
MAPK1 (ERK2) is a serine/threonine kinase that activates p90 RSK, MSK, ELK1, and Stat3, and plays a key role in the MAPK signaling pathway.
MAP kinases (ERKs) integrate multiple biochemical signals and regulate diverse cellular processes such as proliferation, differentiation, transcription, and development.
Activation of MAPK1 requires phosphorylation by upstream kinases, after which it translocates to the nucleus to phosphorylate nuclear targets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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