Thermo Fisher Scientific CD19 Monoclonal Antibody (MB19-1), FITC, eBioscience
마우스 CD19를 인식하는 MB19-1 단클론 항체로, FITC 형광 표지되어 흐름세포분석에 적합합니다. B세포 발현 분석용으로 사용되며, 488 nm 여기/520 nm 방출 특성을 가집니다. 액상 형태로 4°C 암소 보관, 동결 금지.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Paraffin) (IHC-P) | - | 1 publication |
| Flow Cytometry (Flow) | 1 µg/test | 16 publications |
| Neutralization (Neu) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgA, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MB19-1 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_464965 |
Additional Formats
- APC
- Biotin
- Functional Grade
- PE
- Custom conjugation available
Product Specific Information
The MB19-1 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein expressed by B cells throughout development, excluding plasma cells. Follicular dendritic cells also express CD19. CD19 forms a multimolecular complex with CD21, CD81, and MHC class II, associating with the B cell receptor (BCR). CD19 signaling induces tyrosine phosphorylation, calcium flux, and B cell proliferation. Staining with MB19-1 is typically dimmer than that with the rat anti-mouse CD19 clone 6D5.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes; ≤1 µg/test recommended.
A test is defined as the amount of antibody sufficient to stain cells in 100 µL final volume. Cell number may range from 10⁵–10⁸ cells/test. Optimize titration for best performance.
Fluorescence Characteristics:
- Excitation: 488 nm
- Emission: 520 nm
- Laser: Blue
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on all peripheral B cells and some myeloid leukemia cells. CD19 acts as a coreceptor with the B cell receptor (BCR), lowering the threshold for activation and amplifying antigen receptor signaling. It plays a central role in regulating normal and malignant B cell proliferation. Mutations can cause hypogammaglobulinemia, while overexpression leads to B cell hyperactivity.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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