Thermo Fisher Scientific TagRFP Recombinant Rabbit Monoclonal Antibody (JA10-52)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Published species

Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JA10-52

Immunogen

Recombinant protein within Entacmaea quadricolor eqFP611 RFP aa 1-216

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2809945

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Red fluorescent protein (TagRFP) derived from Entacmaea quadricolor eqFP611 (Uniprot ID: Q8ISF8) is an important tool in biotechnology and cell biology as a spectrally distinct companion or substitute for the green fluorescent protein (GFP; from Aequorea jellyfish). Pigment protein. The emission does not change over the pH range of 4 to 10. Maturation occurs via a green intermediate and is complete within 12 hours. Recombinant expression at high temperatures induces oligomerization. Abs(max)=559 nm. Exhibits a smaller absorbance peak at 525 nm. Has a strong fluorescence emission spectrum which peaks at 611 nm. [Uniprot]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.