Thermo Fisher Scientific TagRFP Recombinant Rabbit Monoclonal Antibody (JA10-52)
TagRFP 단백질을 인식하는 재조합 토끼 단클론 항체로 높은 특이성과 재현성을 제공합니다. Western blot 및 IHC에 적합하며, 로트 간 일관성과 민감도가 우수합니다. 연구용으로만 사용되며 장기 보관 시 -20°C에서 보관하십시오.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | - |
| Immunohistochemistry (IHC) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JA10-52 |
| Immunogen | Recombinant protein within Entacmaea quadricolor eqFP611 RFP aa 1–216 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809945 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, followed by screening to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
Red fluorescent protein (TagRFP) derived from Entacmaea quadricolor eqFP611 (Uniprot ID: Q8ISF8) is widely used in biotechnology and cell biology as a spectrally distinct companion or substitute for green fluorescent protein (GFP; from Aequorea jellyfish).
Key properties:
- pH stability from 4 to 10
- Maturation via a green intermediate within 12 hours
- High-temperature expression induces oligomerization
- Abs(max): 559 nm
- Secondary absorbance peak: 525 nm
- Strong fluorescence emission peak: 611 nm
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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