Thermo Fisher Scientific Phospho-Histone H3 (Ser10) Polyclonal Antibody
Phospho-Histone H3 (Ser10)를 인식하는 rabbit polyclonal antibody로, WB, ICC, ELISA, IP, ChIP 등 다양한 응용에 사용 가능. 액상 형태이며, -20°C 보관. 세포주기 연구 및 염색질 변형 분석에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| ELISA | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 1:500 |
| ChIP assay (ChIP) | 1–10 µg / 1×10⁴ cells |
| Peptide Array (Array) | – |
| Dot blot (DB) | 1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Raised against histone H3 containing the phosphorylated serine 10 (H3S10p), using a KLH-conjugated synthetic peptide |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | <0.1% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Dry ice |
| RRID | AB_2533890 |
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the nucleosome structure. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translational modifications of histones, such as methylation, acetylation, and phosphorylation, influence chromatin structure and gene expression. The N-terminal tail of histone H3 undergoes these modifications, impacting diverse cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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