Thermo Fisher Scientific H3K36me2 Polyclonal Antibody
히스톤 H3의 Lys36 이메틸화(H3K36me2)를 인식하는 Rabbit Polyclonal 항체로, WB, ICC/IF, ELISA, ChIP, ChIP-Seq, Dot blot 등 다양한 응용에 적합. 액상 형태로 공급되며, -20°C 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:500 |
| ELISA | 1:1,000 |
| ChIP assay (ChIP) | 1–10 µg/1×10⁴ cells |
| ChIP-sequencing (ChIP-Seq) | 0.5 µL/1×10⁶ cells |
| Dot blot (DB) | 1:100,000 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Raised against the region of histone H3 containing dimethylated lysine 36 (H3K36me2), using a KLH-conjugated synthetic peptide. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Lot-Specific |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Dry ice |
| RRID | AB_2533888 |
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. Together with four core histone proteins, it binds to DNA to form the nucleosome structure. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
Post-translational modifications of histones can directly alter chromatin structure or facilitate the binding of specific transcription factors. The N-terminal tail of histone H3 extends from the nucleosome core and can undergo several modifications such as methylation, acetylation, and phosphorylation, which influence various cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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