Thermo Fisher Scientific CD38 Monoclonal Antibody (HB7), PE-Cyanine7, eBioscience

Thermo Fisher Scientific CD38 Monoclonal Antibody (HB7), PE-Cyanine7, eBioscience™

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 5 µL (0.125 µg)/test
  View 6 publications

Product Specifications

Additional Formats Available

• eFluor 450
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 660
• NovaFluor Yellow 755
• PE
• PerCP-eFluor 710
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The HB7 monoclonal antibody reacts with the human CD38 molecule, an approximately 45 kDa type II transmembrane protein. CD38 acts as an ectoenzyme catalyzing NAD into NAADP and cyclic ADP-ribose (cADPR), both secondary messengers.
Expression of CD38 varies during B cell development and is also found in T cells, NK cells, and monocytes. It can be used to phenotype leukemias and monitor HIV-1 progression. The CD34⁺CD38⁻ hematopoietic stem cell population defines the most pluripotent cells. CD38 is also detected in the nucleus where it may regulate calcium levels.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated for flow cytometry using human peripheral blood cells.
Use 5 µL (0.125 µg) per test (100 µL total volume). Cell counts typically range from 10⁵–10⁸ cells/test.

Light Sensitivity: Protect vial and stained samples from light (photo-induced oxidation).
Fixation: Samples may be stored in IC Fixation Buffer (Product #00-822-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark. Clone-specific performance should be verified empirically.
Excitation: 488–561 nm
Emission: 775 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD38 (NAD⁺ glycohydrolase) is a type II transmembrane glycoprotein involved in activation, proliferation, and differentiation of lymphocytes, and apoptosis of progenitor cells.
Functions include ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase, and NAD⁺ glycohydrolase activity, as well as acting as a cell surface receptor.
Antibodies to CD38 are used in subtyping lymphomas and leukemias, detecting plasma cells (myeloma identification), and marking activated B and T cells.
CD38 participates in cell adhesion, signal transduction, and calcium signaling. It is highly expressed in pancreas, liver, kidney, malignant lymphoma, and neuroblastoma.
Diseases associated with CD38 dysfunction include chronic lymphocytic leukemia and Richter’s Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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