Thermo Fisher Scientific MRPS12 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:500

Product Specifications

Product Specific Information

Antibody detects endogenous levels of total MRPS12.

Target Information

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and assist in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit, with approximately 75% protein to rRNA composition, unlike prokaryotic ribosomes where this ratio is reversed. Mammalian mitoribosomes lack a 5S rRNA. The MRPS12 gene encodes a 28S subunit protein belonging to the ribosomal protein S12P family, playing a key role in decoding fidelity and susceptibility to aminoglycoside antibiotics. The mitochondrial seryl-tRNA synthetase gene is located upstream and adjacent to MRPS12, both being possible candidates for the autosomal dominant deafness gene (DFNA4). Splice variants differing in the 5' UTR have been identified, all encoding the same protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.