Thermo Fisher Scientific CD235a (Glycophorin A) Monoclonal Antibody (10F7MN), NovaFluor Blue 510, eBioscience

Thermo Fisher Scientific CD235a (Glycophorin A) Monoclonal Antibody (10F7MN), NovaFluor Blue 510

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 5 µL (0.8 µg)/test

Product Specifications

Additional Formats

• Brilliant UV 661
• Brilliant UV 737
• FITC
• NovaFluor Blue 585
• Custom conjugation available

Product Specific Information

The monoclonal antibody 10F7MN recognizes human glycophorin A (CD235a), detecting both M and N alleles. Glycophorin A is a 151 amino acid sialoglycoprotein found on erythrocyte and erythroid progenitor cell membranes (~500,000 copies per cell). The gene resides on chromosome 4 with two allelic forms (M and N) differing at two amino acids:

• M group: Ser1, Gly5
• N group: Leu1, Glu5

Studies suggest that toxin exposure may cause allele mutations, potentially linked to cancer and heart disease predisposition.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells at 5 µL (0.8 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining.
  • Add 5 µL per sample before mixing with cells.
  • For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology based on nucleic acid dye structures, engineered for optimized fluorescent signatures minimizing spillover and spread.

• Excitation: 496 nm
• Emission: 511 nm
• Laser: 488 nm (Blue)

Target Information

CD235a (Glycophorin A, GPA) is a single-pass membrane sialoglycoprotein expressed in mature erythrocytes and erythroid precursor cells. It carries blood group M and N specificities and provides a mucin-like barrier minimizing red blood cell aggregation. CD235a also acts as a receptor for Sandei virus, parvovirus, and Streptococcus adhesin (Hsa).

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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