Thermo Fisher Scientific TNFA Monoclonal Antibody (OTI5A11), TrueMAB
인간 TNFα를 인식하는 단클론 항체로, Western blot과 Immunocytochemistry에 최적화됨. HEK293T 세포에서 생산된 full-length 재조합 단백질을 면역원으로 사용. 동결건조 형태, PBS 버퍼에 8% 트레할로스 포함. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5A11 |
| Immunogen | Full length human recombinant protein of human TNFA produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
TNF alpha is a multifunctional proinflammatory cytokine belonging to the tumor necrosis factor (TNF) superfamily. It is mainly secreted by macrophages and binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha regulates immune responses, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. It exists as multimers but shows a single 17 kDa band under non-reducing SDS-PAGE. TNF alpha has roles in immune defense, inflammation, and tumor necrosis, and is implicated in autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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