Thermo Fisher Scientific TGF alpha Chimeric Recombinant Rabbit Monoclonal Antibody (tAb2)

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1:100

-

ELISA (ELISA)

Assay-dependent

-

Dot blot (DB)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

tAb2

Immunogen

Biologically active hemocyanin-coupled human TGFalpha.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3074449

Product Specific Information

Specificity: tAb2 binds the N-terminal sequence of transforming growth factor alpha, VVSHFND (with residues 4 to 7 being the minimal binding epitope).

Target Information

TGF-alpha is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF-alpha induces anchorage-independence transformation in cultured cells. Human, murine and rat TGF-alpha are cross-species reactive. TGFalpha (aa 50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.