Thermo Fisher Scientific CD142 Monoclonal Antibody (HTF-1), APC, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HTF-1

Conjugate

APC APC APC

View additional formats

• Functional Grade
• PE
• Request custom conjugation

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11043550

Product Specific Information

Description: This HTF-1 monoclonal antibody reacts with human CD142, which is also known as Tissue Factor. Expression of this type I transmembrane glycoprotein on endothelial cells, monocytes, macrophages, and platelets can be induced by inflammatory mediators (e.g., LPS, IL-1b, TNFa, PMA, or endotoxin). On the other hand, CD142 is expressed constitutively by some tumor cells (e.g., lung, pancreatic, breast, and colon) and non-immune tissues such as the vasculature, central nervous system, kidney, epithelia, and placenta. Studies have also suggested that CD142 exists as a soluble form that circulates in blood. CD142 initiates blood coagulation by associating with and activating the circulating factors VII and VIIa.

The HTF-1 antibody has been reported to exhibit blocking activity.

Applications Reported: This HTF-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HTF-1 antibody has been pre-titrated and tested by flow cytometric analysis of LPS-stimulated normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.