Thermo Fisher Scientific Leptin Receptor Polyclonal Antibody
Leptin 수용체(OB-R)를 인식하는 Thermo Fisher Scientific의 폴리클로날 항체. Western blot, IHC, Flow cytometry 등 다양한 응용에 사용 가능. 인간, 마우스, 랫트 반응성. 합성 펩타이드 항원 기반으로 제작, 0.5 mg/mL 농도의 액상 형태로 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | View 2 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 4–6 µg/mL | – |
| Immunohistochemistry (Frozen) (IHC (F)) | – | View 1 publication |
| Flow Cytometry (Flow) | 10 µg/mL | – |
| Immunoprecipitation (IP) | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (TQDDIEKHQSDAG) corresponding to internal amino acids of LEPR (aa 829–841) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10977531 |
Product Specific Information
- Predicted to react with canine based on sequence homology.
- Tested in Peptide ELISA: antibody detection limit dilution 1:128,000.
Target Information
The leptin (OB-R) receptor is a member of the cytokine receptor superfamily and plays a key role in mammalian body weight homeostasis and energy balance. Activation by leptin triggers downstream signaling through the JAK/STAT pathway. Several isoforms of OB-R exist, differing in length and signaling capability. The long form is expressed in the choroid plexus and hypothalamus, proposed as centers for satiety and energy expenditure.
Mutations in mouse OB-R (db) or leptin (ob) genes lead to early-onset obesity and metabolic abnormalities such as hyperinsulinemia, insulin resistance, and increased adipose tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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