Thermo Fisher Scientific Leptin Receptor Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

4-6 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Flow Cytometry (Flow)

10 µg/mL

-

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (TQDDIEKHQSDAG) corresponding to the internal amino acids of LEPR (aa 829-841). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Leptin Receptor,uniProtId:P48357-1,ncbiNodeId:9606,antigenRange:829-841,antigenLength:1165,antigenImageFileName:PA5-18522_Leptin_Receptor_P48357-1_Goat.svg,antigenImageFileNamePDP:PA5-18522_Leptin_Receptor_P48357-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_10977531

Product Specific Information

This antibody is predicted to react with canine based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.

Target Information

The leptin (OB-R) receptor is a member of the cytokine receptor superfamily which plays an important role in mammalian body weight homeostasis and energy balance. Ligand-induced activation of the OB-R by the adipose tissue-secreted hormone, leptin, appears to activate downstream signaling events through the Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway. Leptin can homo-dimerize OB-R extracellular domains while downstream signaling events are initiated upon homo-dimerization of OB-R intracellular domains. Several isoforms of the OB-R which differ in length and signaling capabilities have been reported. RNA transcripts encoding the OB-R long form have been found in the choroid plexus and in the hypothalamus which has been proposed as a control center for satiety and energy expenditure. Mutations in either the mouse OB-R (db) or leptin (ob) genes have been shown to result in early-onset obesity. Metabolic abnormalities attributable to these genotypes include hypercorticoidemia, hyperinsulinemia and insulin resistance, hyperglycemia, altered CNS activity, reduced metabolic rate of brown adipose tissue, and a large increase in white adipose tissue.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.