Thermo Fisher Scientific beta Amyloid Monoclonal Antibody (DE2B4)
인간 베타 아밀로이드(β-Amyloid)를 인식하는 Mouse IgG1 단클론 항체(DE2B4). Western blot, IHC, ICC, ELISA, IP에 사용 가능. 알츠하이머병 관련 연구용으로 적합하며, Protein A 정제 및 PBS 버퍼에 0.09% sodium azide 함유. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution / Condition |
|---|---|
| Western Blot (WB) | 2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:200 |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | DE2B4 |
| Immunogen | Synthetic peptide corresponding to residues 1–17 of human beta Amyloid |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10978144 |
Product Specific Information
- Protein digestion pretreatment of paraffin sections using formic acid for 2–3 minutes is required prior to staining with MA1-24966.
- Positive Control: Alzheimer’s disease brain
Target Information
Amyloid beta peptide (Abeta/Beta-amyloid) is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer’s disease.
Abeta peptide (40–43 amino acids) is generated from the beta-amyloid precursor protein (beta APP) through sequential cleavage by beta-secretase (BACE) and gamma-secretase.
Gamma-secretase, a multi-subunit complex containing presenilin-1 and -2, processes the membrane-bound remnant of beta APP to produce the amyloid beta peptide.
Abeta is an extracellular filamentous protein found in amyloid cores, neuritic plaques, and neurofibrillary tangles.
Beta amyloid deposits are associated with Alzheimer’s disease, Lewy body dementia, Down’s syndrome, Dutch-type amyloidosis, cerebral amyloid angiopathy, and the Guam Parkinson-Dementia complex.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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