Thermo Fisher Scientific IL-23 p19 Antibody Cocktail, eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

The antibody eBio473P19 reacts with the p19 subunit of human IL-23. It was generated by immunization with recombinant human IL-23 heterodimer expressed in insect cells.

Using a p19-specific capture antibody and a p40-specific detection antibody enables a highly specific human IL-23 sandwich ELISA. No cross-reactivity with IL-12 p40 monomer, IL-12 p70, or 20 unrelated cytokines was observed. The assay detects native human IL-23 protein in supernatants from activated dendritic cell populations.

IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a p19 protein. The p19 subunit is inactive by itself. IL-23 interacts with IL-12Rβ1 and a novel β2-like receptor subunit (IL-23R) containing a STAT4 binding domain. It is secreted by activated mouse and human dendritic cells.

Mouse IL-23 differs from IL-12 in biological activity. It does not induce significant IFN-γ production but promotes proliferation of memory T cells and production of IL-17. Human IL-23 induces proliferation of memory T cells and moderate IFN-γ production.

Applications Reported

• Immunoblotting (WB)
• ELISA

Applications Tested

• Capture antibody in sandwich ELISA for human IL-23 (p19p40) detection, combined with biotinylated p40-specific antibody (C8.6, Cat. 13-7129) and recombinant IL-23 standard (Cat. 14-8239)
• Recommended ELISA capture concentration: 2.5–8 µg/mL
• Standard curve: 2000 pg/mL – 15 pg/mL
• Use TMB substrate (not ABTS)
• Effective in immunoblotting under reducing and nonreducing conditions at 2 µg/mL

*eBio473P19 is a cocktail of two clones (eBio11P19 and eBio25P19) at 0.5 mg/mL each.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with p19. IL-23 interacts with IL-12Rβ1 and IL-23R to mediate its biological effects. It promotes proliferation of memory T cells and production of IL-17, distinguishing it from IL-12, which primarily induces IFN-γ production.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.