Thermo Fisher Scientific AKAP associated Sperm Protein Polyclonal Antibody
AKAP associated Sperm Protein(ROPN1L)을 인식하는 Rabbit Polyclonal Antibody로, Human, Mouse, Rat에 반응합니다. WB, IHC, ICC, ELISA 등 다양한 응용에 적합하며, Protein A 정제 및 고순도 유지. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | References |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | - |
| ELISA | 1:500–1:1,000 | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human ASP/AKAP associated Sperm Protein (amino acids 21–100) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Principal Names: ROPN1L; Ropporin-1 like; ASP; AKAP-associated sperm protein
Official Gene Symbol: ROPN1L
Gene ID: 83853 (human)
Gene Map Locus: 5p15.2 (human)
AKAPs act as scaffolding proteins coordinating phosphatase/kinase signaling at the cytoskeleton. They interact with PKAs and anchor them to specific sub-cellular compartments, participating in multiple cellular processes including growth, metabolism, and differentiation.
ASP (26 kDa) is a novel AKAP-interacting protein that binds to the amphipathic helix of AKAP3 and facilitates AKAP3–PKA interactions. It contains a conserved N-terminal AKAP-binding domain and a central cysteine-rich Fz domain. It is specifically expressed in sperm and may play a significant role in sperm motility and cytoskeletal reorganization during spermiogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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