Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (A1exF5), Alexa Fluor 700, eBioscience

Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (A1exF5), Alexa Fluor 700, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Alexa Fluor 488
• APC
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 650
• Brilliant Violet 711
• eFluor 450
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody A1exF5 recognizes both human and mouse Arginase 1 (Arg1), a cytosolic enzyme.
This clone is compatible with standard intracellular staining protocols and the Foxp3/Transcription Factor Staining Buffer Set.

Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometry using stimulated mouse bone marrow cells with Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00).
Recommended use ≤ 0.125 µg per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test.
Careful titration recommended for optimal performance.

Fluorochrome Information:
Alexa Fluor® 700 emits at 723 nm, excited by red laser (633 nm).
Typical filter setup: 685 LP mirror and 710/20 filter.
Excitation: 633–647 nm; Emission: 723 nm; Laser: Red Laser.

Target Information

Arginase-1 (Arg1) is a 35 kDa enzyme that converts L-arginine to urea and L-ornithine, the final step in the urea cycle.
It regulates nitric oxide production by depriving NO synthase of its substrate.
Arginase 1 is expressed in:

• Liver
• Neutrophils
• Myeloid-derived suppressor cells (MDSC)
• Neural stem cells

In humans, expression occurs in blood neutrophils but not CCR3+ granulocytes.
In mice, it marks alternatively activated macrophages (M2a).
Arginase-1 is also found in myeloid cells infiltrating tumors and in hepatocellular carcinoma.
Defects cause argininemia, an autosomal recessive disorder with hyperammonemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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