Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (A1exF5), Alexa Fluor 700, eBioscience
인간 및 생쥐 Arginase 1을 인식하는 단클론 항체로 Alexa Fluor 700으로 표지됨. Flow cytometry에 최적화되어 있으며 세포내 염색 프로토콜과 호환. 696/719 nm 발광 특성으로 적색 레이저 검출 가능. 연구용으로만 사용.
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Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (A1exF5), Alexa Fluor 700, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.125 µg/test | View 21 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 700 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A1exF5 |
| Immunogen | E.coli-derived Recombinant mouse Arginase 1 |
| Conjugate | Alexa Fluor™ 700 |
| Excitation / Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2734832 |
Additional Formats Available
- Alexa Fluor 488
- APC
- Brilliant UV 805
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 650
- Brilliant Violet 711
- eFluor 450
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The monoclonal antibody A1exF5 recognizes both human and mouse Arginase 1 (Arg1), a cytosolic enzyme.
This clone is compatible with standard intracellular staining protocols and the Foxp3/Transcription Factor Staining Buffer Set.
Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometry using stimulated mouse bone marrow cells with Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00).
Recommended use ≤ 0.125 µg per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test.
Careful titration recommended for optimal performance.
Fluorochrome Information:
Alexa Fluor® 700 emits at 723 nm, excited by red laser (633 nm).
Typical filter setup: 685 LP mirror and 710/20 filter.
Excitation: 633–647 nm; Emission: 723 nm; Laser: Red Laser.
Target Information
Arginase-1 (Arg1) is a 35 kDa enzyme that converts L-arginine to urea and L-ornithine, the final step in the urea cycle.
It regulates nitric oxide production by depriving NO synthase of its substrate.
Arginase 1 is expressed in:
- Liver
- Neutrophils
- Myeloid-derived suppressor cells (MDSC)
- Neural stem cells
In humans, expression occurs in blood neutrophils but not CCR3+ granulocytes.
In mice, it marks alternatively activated macrophages (M2a).
Arginase-1 is also found in myeloid cells infiltrating tumors and in hepatocellular carcinoma.
Defects cause argininemia, an autosomal recessive disorder with hyperammonemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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