Thermo Fisher Scientific IFN gamma Monoclonal Antibody (GZ-4), eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 1 publication 1 publication

Functional Assay (Functional)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

GZ-4

Immunogen

Rec. Human IFN gamma

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• FITC
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.2-7.4

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10609338

Product Specific Information

Description: Anti-human IFN-gamma (GZ-4) specifically recognizes natural IFN-gamma from human T-lymphocytes as well as recombinant human IFN-gamma produced in E.coli.

BMS107FI is suitable for intracellular staining of IFN-gamma, as primary antibody in staining for FACS analysis, and can be used for direct staining of cells.

BMS107BT can be used in a 2-step procedure using the biotin-(strept)avidin system.

BMS107 can be used as a neutralizing agent.

In a bioassay using inhibitionof the cytopathic encephalomyocarditis virus effect on human lung carcinoma cell line A459, it has been determined that less than 400 ng of BMS107 are necessary to neutralize the antiviral activity of 1 Unit IFN-gamma

BMS107 does not cross react with human IFN-alpha, IFN-beta and IFN-omega. Cross reacts with primates (African Green Monkeys).

Applications Tested: Flow Cytometry (Intracellular Staining), Functional Studies (Blocking).

Purity: >95%.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.